Participants will gain an understanding about the strategies and the rationale for the development of a separation of enantiomers. The course will provide a unique way of approaching method development for separating enantiomers – one that is based upon the intimate interaction between the analytes and the chiral stationary phase.  This approach avoids “trial and error” methods and will enable participants to more effectively and efficiently pursue method development for the separation of enantiomers.

1. Main Types of Molecular Interactions leading to Enantiomeric Separation and Thermodynamics Aspects of Enantiomeric Separation
   • H-Bonds
   • Inclusion complexes
   • Charge transfer
     
     This section will bring in the main interactions leading to chiral separation, i.e. through the formation of hydrogen bonds, inclusion compounds, and charge transfer.  Each type of interaction will be illustrated with examples which demonstrate separation through a particular type of transient diastereomeric species.
      
2. Mixed types of interactions
   • Polysaccharide Phases
   • Antibiotic Phases
   • Protein Phases
     
     This section will present the types of stationary phases exhibiting a combination of interactions (i.e. hydrogen bonds and inclusion, hydrogen bonds and charge transfer, etc.).   Chiral phases such as antibiotic phases, polysaccharide phases, and protein phases will be discussed, along with examples of separation using such stationary phases. 
     
3. Ligand Exchange
   The concept of enantiomeric separation through the formation of transient diastereomeric complexes with transitional metals will be presented.   The mechanism to form such complexes will be presented and examples of separations using this approach will be provided
   
4. Strategies for method development in chiral separation
   In this section, the strategies for developing a separation of enantiomers based on knowledge gained  in previous sections will be presented. 

Dr. Nelu Grinberg is a Distinguished Scientist in the Chemical Development Department at Boehringer Ingelheim Pharmaceuticals in Ridgefield, CT.  Prior to this, he worked for sixteen years in the Analytical Department at Merck Research Laboratories in Rahway, NJ, where he was a Senior Research Fellow. He has worked in the field of chiral separation for more than twenty years and has published intensively in this area.  He has authored and coauthored over 90 publications, including articles and book chapters.  He is currently on the editorial board of the Journal of Liquid Chromatography and Related Techniques and is a Co-editor of the Advances in Chromatography series. He was the Course Director for a Short Course on Separation of Enantiomers at the Eastern Analytical Symposium (Somerset, NJ).  He is the President of Connecticut Separation Science Council, and member of the Board of Directors of the Society of Small Molecule Science.  Dr. Grinberg obtained his Ph.D. in Chemistry from the Technical University of Iasi in Romania. He conducted post¬doctoral research with Professor Barry Karger at Northeastern University and with Professor E. Gil-Av at The Weizmann Institute of Science in Rehovot, Israel.